Replacing Animal Models - A Practical Guide toCreating and Using Culture-based BiomimeticAlternatives
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More About This Title Replacing Animal Models - A Practical Guide toCreating and Using Culture-based BiomimeticAlternatives


Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments.

The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing.

The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.


Professor Jamie A. Davies, Centre for Integrative Physiology, Hugh Robson Building, George Square, University of Edinburgh, Edinburgh.


Contributors, vii

Preface, xi

Section 1 Introductory Material

1 Potential Advantages of Using Biomimetic Alternatives, 3
Jamie Davies

2 Overview of Biomimetic Alternatives, 13
Jamie Davies

Section 2 Culture Methods

3 Pancreatic Islets, 23
Eli C Lewis

4 Endometrial Organoid Culture, 35
Merja Blauer

5 Modelling Lymphatic and Blood Capillary Patterning, 45
Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel

6 Precision-cut Lung Slices (PCLS), 57
Christian Martin and Stefan Uhlig

7 Human Colon Tissue in Organ Culture, 69
Michael K Dame and James Varani

8 Fetal Organ Culture, 81
Jamie Davies

9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89
Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter

10 Magnetic Assembly of Tissue Surrogates, 107
Chien-Yu Fu and Hwan-You Chang

11 Assembly of Renal Tissues by Cellular Self-organization, 115
Mathieu Unbekandt

Section 3 Case Studies of Use

12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125
Guangping Tai and Jamie Davies

13 Lung Organoid Culture to Study Responses to Viruses, 137
Wenxin Wu, J Leland Booth and Jordan P Metcalf

14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151
James Varani

15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159
Alastair J Sloan, Sarah Y Taylor and Emma L Smith

16 Three-dimensional, High-density and Tissue Engineered Culture Models of Articular Cartilage, 167
Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei

17 Concluding Remarks, 193
Jamie Davies

Appendix 1 Sources of funding for development of culture-based alternatives, 195

Appendix 2 Databases and web-based discussions relevant to development of alternatives, 197

Index, 199