Title Details

Contained in this text are 18 laboratory projects that explore the structural, biochemical and physiological nature of eukaryotic cells. Topics are largely traditional; however, several investigations employ new methodologies. Extended coverage of biochemistry is offered, and materials have been selected for availability and ease of handling: eg. extraction of DNA and RNA done with calf liver; succinate dehydrogenase activity studied in mitochondria isolate from cauliflower. There is more procedural detail than found in most laboratory manuals, negating the need for constant instructional details. A variety of methodologies are introduced, such as cytochemistry, spectrophotometry, electrophoresis, cell fractionation, silver staining of active sites of RNA transcription and many more.