Tissue In Situ Hybridization: Methods in Animal Development
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More About This Title Tissue In Situ Hybridization: Methods in Animal Development
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Principle of the Technique.
Probe Synthesis.
Fixation.
Hybridization to Whole-Mount Embryos.
Immunolocalization of Probes.
Alternative Enzyme Substrates.
Two-Color In Situ Hybridizations.
Two-Color In Situ Hybridization with Fluorochromes.
Embedding and Sectioning Whole-Mount Embryos.
In Situ Hybridization on Paraffin Wax Embedded Tissue Sections.
In Situ Hybridization on Cryostat Sections of Chick Embryos.
Whole-Mount In Situ Hybridization of Thick, Floating Tissue Sections.
Simultaneous Localization of Transcription and Translation Gene Products.
Special Considerations, Modifications, and Problem Troubleshooting.
Photomicrography.
Solutions, Reagents, Equipment, and Suppliers.
References.
Index.
