Natural Products Analysis: Instrumentation, Methods, and Applications
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More About This Title Natural Products Analysis: Instrumentation, Methods, and Applications


This book highlights analytical chemistry instrumentation and practices applied to the analysis of natural products and their complex mixtures, describing techniques for isolating and characterizing natural products.

• Applies analytical techniques to natural products research – an area of critical importance to drug discovery
• Offers a one-stop shop for most analytical methods: x-ray diffraction, NMR analysis, mass spectrometry, and chemical genetics
• Includes coverage of natural products basics and highlights antibacterial research, particularly important as efforts to combat drug resistance gain prominence
• Covers instrumental techniques with enough detail for both current practitioners and beginning researchers


Vladimir Havlicek is a Professor of Analytical Chemistry in the Institute of Microbiology of the Academy of Sciences of the Czech Republic. His laboratory uses analytical tools to characterize molecular structure. He has worked in the field of natural products for over 20 years. Dr. Havlicek is on the editorial or advisory boards of several leading analytical journals and has published over 130 papers in peer-reviewed journals.

Jaroslav Spizek is a Professor of Molecular and Cell Biology and Genetics in the Institute of Microbiology of the Academy of Sciences of the Czech Republic, where he was director between1992-2000. He has worked in the field of antibiotics for 50 years. In 2011, he became an Elected Fellow of the American Academy of Microbiology. Professor Spizek has published 98 peer-reviewed papers and edited two books.


Preface ix

Contributors xi

1. Natural Products Analysis: Instrumentation, Methods, and Applications 1
Vladimír Havlíèek and Jaroslav Spí?ek

2. The Need for New Antifungal and Antimalarial Compounds 9
Jaroslav Spí?ek and Arnold L. Demain

3. Emerging Instrumental Methods for Antimicrobial Resistance and Virulence Testing 25
Plamen A. Demirev

4. Plant and Marine Sources: Biological Activity of Natural Products and Therapeutic Use 43
Amedeo Amedei and Elena Niccolai

5. Emerging Trends for Stimulating the Discovery of Natural Products 115
Navid Adnani, Gregory A. Ellis, Thomas P. Wyche, Tim S. Bugni, Jason C. Kwan, and Eric W. Schmidt

6. Advances and Challenges in Optical Molecular Spectroscopy Including Surface Plasmon Resonance-Based Methods for Bioanalysis 163
Pavel Matìjka, Blanka Vlèková, Lucie Bednárová, and Petr Malon

7. Advanced Techniques for NMR Analysis of Complex Biological Mixtures—From Simple NMR to Hyphenated Techniques 239
Helena Pelantová, Simona Bártová, and Marek Kuzma

8. Advances in X-Ray Diffraction: Implications to the Pharmaceutical Industry 285
Alexandr Jegorov and Michal Hušák

9. Laser Ablation Inductively Coupled Plasma Mass Spectrometry as a Tool in Biological Sciences 313
Michaela Vašinová Galiová, Jan Havliš, and Viktor Kanický

10. Imaging Mass Spectrometry, Metabolism, and New Views of the Microbial World 349
B. Christopher Hoefler and Paul D. Straight

11. Structural Separations for Natural Product Characterization by Ion Mobility–Mass Spectrometry: Fundamental Theory to Emerging Applications 397
Sarah M. Stow, Nichole M. Lareau, Kelly M. Hines, C. Ruth McNees, Cody R. Goodwin, Brian O. Bachmann, and John A. McLean

12. High-Resolution Tandem Mass Spectrometry for Nonribosomal Peptide and Polyketide Analysis 433
Rebecca H. Wills, Manuela Tosin, and Peter B. O’Connor

13. Natural Product Drug Discovery and Analysis Using Mass Spectrometry and Affinity-Based Technologies 475
Evelyn H. Wang and Kevin A. Schug

14. Glycosylated Ribosomally Synthesized Peptide Toxins: Discovery, Characterization, and Applications 507
Gillian E. Norris and Mark L. Patchett

15. Using Ultrahigh-Resolution Mass Spectrometry to Unravel the Chemical Space of Complex Natural Product Mixtures 545
Constanze Müller, Mourad Harir, Norbert Hertkorn, Basem Kanawati, Dimitrios Tziotis, and Philippe Schmitt-Kopplin

16. Functional Amyloid Fibrils: Lessons from Microbes 571
Sally L. Gras and Dennis Claessen

Index 601


“Every chapter, even those that depart somewhat from the declared remit, is packed with information most of it, as I said at the beginning, representing the cutting edge of the subject.  I cannot recommend it too highly.”  (Chromatographia, 1 May 2015)