Accessing Uncultivated Microorganisms
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More About This Title Accessing Uncultivated Microorganisms

English

A complete overview of methods and approaches to studying microbial diversity.

Emphasizes new innovative methods, addresses established methods, and provides “hands on” advice on how to isolate, cultivate, and study organisms.Covers the range of assessment possibilities from direct measurements of physical, chemical, and biological aspects of the environment to a wide array of molecular tools such as metagenomic and metaproteomic approaches. Details innovative methods such as the use of genetic information from environmental samples, enrichment cultures, and single cells. Offers practical, essential advice on how to put these methods into practice both within and outside of the laboratory environment.

English

Table of Contents


Part I. Introduction

1. Does Cultivation Still Matter? Karsten Zengler

2. The Human Intestinal Microbiota and Its Impact on Human Health, Mirjana Rajiliæ-Stojanoviæ, Willem M. de Vos, and Erwin G. Zoetendal



Part II. The Status Quo: Who Is Out There? How To Determine Microbial Diversity

3. The Uncountables, William T. Sloan, Christopher Quince, and Thomas P. Curtis

4. The Missing Fungi: New Insights from Culture-Independent Molecular Studies of Soil, S. K. Schmidt, K. L. Wilson, A. F. Meyer, C. W. Schadt, T. M. Porter, and J. M. Moncalvo

5. The Diversity of Free-Living Protists Seen and Unseen, Cultured and Uncultured, David A. Caron and Rebecca J. Gast

6. Microbial Biogeography: Patterns in Microbial Diversity across Space and Time, Noah Fierer

7. The Least Common Denominator: Species or Operational Taxonomic Units? Ramon Rosselló-Mora and Arantxa López-López

8. Measuring Diversity, Jed A. Fuhrman

9. Metagenomics as a Tool To Study Biodiversity, Karen E. Nelson



Part III. Are Microorganisms Nonculturable or not yet Culturable?

10. New Cultivation Strategies for Terrestrial Microorganisms, Peter H. Janssen

11. Cultivation of Marine Symbiotic Microorganisms, Todd A. Ciche

12. Methods To Study Consortia and Mixed Cultures, Boran Kartal and Marc Strous



Part IV. Do We Have To Change Gear? New Cultivation Approaches and New Molecular Approaches Combined

13. Microbial Cell Individuality, Simon V. Avery

14. Nanomechanical Methods To Study Single Cells, Ramya Desikan, Laurene Tetard, Ali Passian, Ram Datar, and Thomas Thundat

15. Single-Cell Genomics, Martin Keller, Christopher W. Schadt, and Anthony V. Palumbo

16. How Many Genes Does a Cell Need? Hamilton O. Smith, John I. Glass, Clyde A. Hutchison III, and J. Craig Venter
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