Molecular Biology and Biotechnology
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More About This Title Molecular Biology and Biotechnology

English

Provides clear, indispensable information in cell and molecular biology that explains the exciting advances in biology and biotechnology.

Serves as both a textbook and a laboratory activities manual. Written in a clear, easy-to-understand language that makes complicated information accessible to those with minimal background in this area. Presents activities that have been field tested, most for more than a decade

English

Table of Contents


Part I. Laying the Foundation



1. An Overview of Biotechnology



2. Cell Properties and Processes



3. Genes, Genetics, and Geneticists



4. An Overview of Molecular Biology



5. Recombinant DNA Technology



Part II. Classroom Activities


A. DNA Structure and Function

6. Student Activity: Constructing a Paper DNA Model

7. Student Activity: DNA Replication

8. Student Activity: From Genes to Proteins
Student Activity: Making Antisense: Regulating Gene Expression with RNA
9. Student Activity: Sizes of the Escherichia coli and Human Genomes

10. Student Activity: Extraction of Bacterial DNA

B. Manipulation and Analysis of DNA

11. Student Activity: DNA Scissors

12. Student Activity: DNA Goes to the Races

13. Student Activity: Restriction Analysis of Lambda DNA

14. Student Activity: Recombinant Paper Plasmids

15. Student Activity: Restriction Analysis Challenge

16. Student Activity: Detection of Specific DNA Sequences: Part I. Fishing for DNA
Student Activity: Detection of Specific DNA Sequences: Part II. Combining Restriction and Hybridization Analysis
Student Activity: Detection of Specific DNA Sequences: Part III. Southern Hybridization
17. Student Activity: Paper PCR

18. Student Activity: DNA Sequencing: The Terminators
Reading: Chain Terminators as Antiviral Drugs
C. Transfer of Genetic Information
Reading: Gene Transfer, Escherichia coli, and Disease
19. Student Activity: Transformation of Escherichia coli

20. Student Activity: Conjugative Transfer of Antibiotic Resistance in Escherichia coli
Reading: Gene Transfer and the Spread of Antibiotic Resistance
21. Student Activity: Transduction of an Antibiotic Resistance Gene

22. Student Activity: Agrobacterium tumefaciens: Nature’s Plant Genetic Engineer

D. Molecular Biology and Genetics

23. Student Activity: An Adventure in Dog Hair, Part I

24. Student Activity: An Adventure in Dog Hair, Part II: Yellow Labs

25. Student Activity: Human Molecular Genetics
Reading: Molecular Genetics of Cancer
26. Student Activity: Genetics in Action

E. Genomics

27. Student Activity: Comparing Genomes
Exercise 1: STRs Can Cause an RFLP
Exercise 2: PCR Can Reveal Differences at Microsatellite Loci
Reading: Mitochondrial DNA

28. Student Activity: Forensic DNA Typing
Exercise 1. A Mix-Up at the Hospital
Exercise 2. A Paternity Case
Exercise 3. The Case of the Bloody Knife
Reading: DNA Forensics: An Archaeological Case

29. Student Activity: Mapping a Disease Gene
Reading: The Human Genome Project: Its Science, Applications, and Issues

30. Student Activity: Microarray Analysis of Genome Expression
Reading: Personal Genomics
F. Bioinformatics and Evolutionary Analysis of Proteins

31. Student Activity: Testing for Amylase Activity

32. Student Activity: Electrophoresis of Amylase Samples

33. Student Activity: Constructing an Amylase Evolutionary Tree

34. Student Activity: Exploring Bioinformatics


Part III. Societal Issues
Introduction to Societal Issues
35. Student Activity: Facing the Forces: Science, Technology, and Society
Reading: The Nature of Science

36. Student Activity: Analyzing the Issues of Biotechnology: Cloning
Reading: Are Bioengineered Foods Safe?

37. Student Activity: The Risks of Technology: Perception, Reality, or Both?
Reading: Biotechnology Regulation in the United States

38. Student Activity: Making Decisions When There is No Right Answer

39. Student Activity: Analyzing Ethical Dilemmas in Genetic Testing
Reading: Science, Law, and Politics
40. Student Activity: Analyzing Ethical Dilemmas in Gene Therapy


Part IV. Appendixes



A. Worksheets



B. Laboratory Biosafety



C. Aseptic Technique



D. Basic Microbiological Methods
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