Molecular Biology and Biotechnology
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More About This Title Molecular Biology and Biotechnology


Provides clear, indispensable information in cell and molecular biology that explains the exciting advances in biology and biotechnology.

Designed for those instructors interested in “problem-based” approaches for teaching and learning.
Includes activities for both wet and dry laboratory settings.Teaches essential critical thinking skills.Offers instructors many valuable teaching implements, including worksheets, templates, and teaching tips, and a companion instructor CD-ROM.


Table of Contents

Part I. Laying the Foundation

1. An Overview of Biotechnology

2. Cell Properties and Processes

3. Genes, Genetics, and Geneticists

4. An Overview of Molecular Biology

5. Recombinant DNA Technology

Part II. Classroom Activities

A. DNA Structure and Function

6. DNA Structure
Student Activity: Constructing a Paper DNA Model

7. DNA Replication
Student Activity: DNA Replication

8. Expression of Genetic Information
Student Activity: From Genes to Proteins
Student Activity: Making Antisense: Regulating Gene Expression with RNA
Advanced Activity: Gene Regulation
Advanced Activity: Antisense and RNA Interference

9. Modeling Genome Size
Student Activity: Sizes of the Escherichia coli and Human Genomes

10. DNA Extraction
Student Activity: Extraction of Bacterial DNA
B. Manipulation and Analysis of DNA

11. Restriction Enzymes
Student Activity: DNA Scissors

12. Gel Electrophoresis
Student Activity: DNA Goes to the Races

13. Restriction Analysis
Student Activity: Restriction Analysis of Lambda DNA

14. Recombinant DNA
Student Activity: Recombinant Paper Plasmids

15. Restriction Analysis Challenge Worksheets
Student Activity: Restriction Analysis Challenge

16. Detection of Specific DNA Sequences: Hybridization Analysis
Student Activity: Detection of Specific DNA Sequences: Part I. Fishing for DNA
Student Activity: Detection of Specific DNA Sequences: Part II. Combining Restriction and Hybridization Analysis
Student Activity: Detection of Specific DNA Sequences: Part III. Southern Hybridization

17. The Polymerase Chain Reaction
Student Activity: Paper PCR

18. DNA Sequencing
Student Activity: DNA Sequencing: The Terminators
Reading: Chain Terminators as Antiviral Drugs
C. Transfer of Genetic Information
Reading: Gene Transfer, Escherichia coli, and Disease
19. Transformation
Student Activity: Transformation of Escherichia coli

20. Conjugation
Student Activity: Conjugative Transfer of Antibiotic Resistance in Escherichia coli
Reading: Gene Transfer and the Spread of Antibiotic Resistance

21. Transduction
Student Activity: Transduction of an Antibiotic Resistance Gene

22. Gene Transfer in Plants by Agrobacterium tumefaciens
Student Activity: Agrobacterium tumefaciens: Nature’s Plant Genetic Engineer
D. Molecular Biology and Genetics

23. Mendelian Genetics at the Molecular Level: Dominance and Recessivity
Student Activity: An Adventure in Dog Hair, Part I

24. Mendelian Genetics at the Molecular Level: An Example of Epistasis
Student Activity: An Adventure in Dog Hair, Part II: Yellow Labs

25. Human Molecular Genetics
Student Activity: Human Molecular Genetics
Reading: Molecular Genetics of Cancer

26. Medical Sleuth: A Story of Genetics in Action
Student Activity: Genetics in Action
E. Genomics

27. Comparing Genomes
Student Activity: Comparing Genomes
Exercise 1: STRs Can Cause an RFLP
Exercise 2: PCR Can Reveal Differences at Microsatellite Loci
Reading: Mitochondrial DNA

28. Forensic DNA Typing
Student Activity: Forensic DNA Typing
Exercise 1. A Mix-Up at the Hospital
Exercise 2. A Paternity Case
Exercise 3. The Case of the Bloody Knife
Reading: DNA Forensics: An Archaeological Case

29. Genome Mapping
Student Activity: Mapping a Disease Gene
Reading: The Human Genome Project: Its Science, Applications, and Issues

30. Microarrays and Genome Analysis
Student Activity: Microarray Analysis of Genome Expression
Reading: Personal Genomics
F. Bioinformatics and Evolutionary Analysis of Proteins

31. Amylase, an Evolutionarily Conserved Enzyme
Student Activity: Testing for Amylase Activity

32. Protein Electrophoresis
Student Activity: Electrophoresis of Amylase Samples

33. Analyzing Evolutionary Changes
Student Activity: Constructing an Amylase Evolutionary Tree

34. Bioinformatics
Student Activity: Exploring Bioinformatics

Part III. Societal Issues

Introduction to Societal Issues

35. Science, Technology, and Society
Student Activity: Facing the Forces: Science, Technology, and Society
Reading: The Nature of Science

36. A Framework for Rational Analysis of Issues
Student Activity: Analyzing the Issues of Biotechnology: Cloning
Reading: Are Bioengineered Foods Safe?

37. Risks and Rationality in Today’s Culture of Fear
Student Activity: The Risks of Technology: Perception, Reality, or Both?
Reading: Biotechnology Regulation in the United States

38. Bioethical Issues: A Decision-Making Model
Student Activity: Making Decisions When There is No Right Answer

39. Bioethics Case Study: Genetic Testing
Student Activity: Analyzing Ethical Dilemmas in Genetic Testing
Reading: Science, Law, and Politics

40. Bioethics Case Study: Gene Therapy
Student Activity: Analyzing Ethical Dilemmas in Gene Therapy

Part IV. Appendixes

A. Worksheets
B. Laboratory Biosafety
C. Aseptic Technique
D. Basic Microbiological Methods